G blocks polypeptide synthesis by inhibiting the elongation step in both prokaryotic and eukaryotic cells. Quality Control: Each lot is thoroughly tested to ensure the absence of lot-to-lot variation. Cell-culture tested: potency validated in Gsensitive and Gresistant mammalian cell lines. Non-cytotoxicity of trace contaminants: absence of long-term effects confirmed in Gresistant cells.
Formula: C 20 H 40 N 4 O G is shipped at room temperature. However, the optimal concentration needs to be determined for your cells. Suggested concentrations of G for selection in some examples of mammalian cells are listed below:. Arikawa K. Ligand-dependent inhibition of B16 melanoma cell migration and invasion via endogenous S1P2 G protein-coupled receptor.
Requirement of inhibition of cellular RAC activity. J Biol Chem. DiLillo DJ. J Immunol. Figueroa K. Selectivity of agonists for the active state of M1 to M4 muscarinic receptor subtypes. J Pharmacol Exp Ther. Kwon JA. For the selection and maintenance of bacterial and eukaryotic cells which have been stably transfected with the E. Susceptibility of cells to hygromycin B varies with cell type, proliferative activity, and other parameters; therefore, we suggest performing a full dose-response curve for each cell system used.
Puromycin is used to select for cell lines that have been transformed by vectors that express puromycin-N-acetyl-transferase pac , e. This is the mg format. For the 25 mg format see Cat. Puromycin supplied in mg and mg formats. Used to select for cell lines that have been transfected with vectors containing the puromycin-N-acetyl-transferase pac resistance gene. This is the 25 mg format. For mg format see Cat. Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications.
Please see the Kit Components List to determine kit components. All Rights Reserved. All trademarks are the property of Takara Bio Inc. Certain trademarks may not be registered in all jurisdictions. Additional product, intellectual property, and restricted use information is available at takarabio.
As an experienced, reliable solutions provider, we offer full-scale customization services for a vast array of reagents and instruments backed by a quality management system, on-time delivery, and dependable support—giving you a competitive advantage in the ever-changing clinical landscape.
Learn how we can catalyze your innovation. Learn more. Create an account to get started. What does it take to generate good science? Careful planning, dedicated researchers, and the right tools. G is specific for the eukaryotic ribosome while the others more specifically inhibit the bacterial organelle.
I believe that G works in bacteria, but at the price I'd rather use kanamycin. Good luck with it! Just out of curiosity Is it just so it selects certain cells? I hope this information can be useful for somebody: "Transfected cells are also selected by resistence to a number of drugs, such as neomycin , its ANALOGUE Geneticin G , hygromycin, and methotrexate, by including a resistence-conferring gene in the construct used for transfection.
Culture in the correct concentration of the selective marker, determined by titration against the transfected and nontransfected controls, selects for stable transfectants. Fourth Edition. Saludos desde Mexico.
0コメント